Consulting and Research Services

· Daniel Kos, Joseph A. Ross, Nick Allan, Mike Jelinski, Stuart Thiessen, Murray Jelinski, Antonio Ruzzini. 2025. Genome sequences of Pasteurellaceae involved in bovine respiratory disease. Microbiology Resource Announcements. e00352-25.

· Chia Hao Ho, Irafasha C. Casmil, Manu Sharma, Tim Rees, Kenza Enright, Nick Allan, Anna K. Blakney. 2025. Laminar fluid ejection device enables high yield and preservation of mRNA and SaRNA LNP formulations. Scientific Reports. 15, 18507 (2025).

· Ann Hammad, Kendall Beaugrand, Joseph A. Ross, Nick Allan, Martin Berman. 2024. Assessing mold growth severity on various textiles: comparative analysis of inoculum densities and fungal strains. Sustainable Microbiology. 2(1):2025.

· Thomas M. Morin, Nick Allan, Joshua Coutts, Jacob M. Hooker, Morgan Langille, Arron Metcalfe, Andrew Thamboo, James Jackson, Manu Sharma, Tim Rees, Kenza Enright, Ken Irving. 2024. Laminar Fluid Ejection for Olfactory Drug Delivery: A Proof of Concept Study. IEEE Journal of Translational Engineering in Health and Medicine. 2024(12):727-738. 

· Steven M. Roche, Brenda J. Ralston, Barbara Olson, Brendan D. Sharpe, Crystal Schatz, Kendall Beaugrand, Joseph A. Ross, Madeleine A. Broomfield, Nicholas Allan, Merle Olson. 2024. Efficacy of a Lidocaine-Impregnated Elastrator Band for Castration and Tail Docking in Lambs. Animals. 14(10):1403.

· Joseph A. Ross, Steven M. Roche, Kendall Beaugrand, Crystal Schatz, Ann Hammad, Brenda J. Ralston, Andrea M. Hanson, Nicholas Allan, Merle Olson. 2024. Assessment of the Effective Tissue Concentrations of Injectable Lidocaine and a Lidocaine-Impregnated Latex Band for Castration in Calves. Animals. 14(6):977.

· Amritha Prasad, Erin Wynands, Steven M Roche, Cristina P. Romo-Bernal, Nicholas Allan, Merle Olson, Sheeny Levengood, Roger Andersen, Nicolas Loebel, Caetano P. Sabino, Joseph A. Ross. 2024. Photodynamic Inactivation of Foodborne Bacteria: Screening of 32 Potential Photosensitizers. Foods. 13(3):453.

· Joseph A. Ross, Steven M. Roche, Kendall Beaugrand, Crystal Schatz, Ann Hammad, Brenda J. Ralston, Andrea M. Hanson, Nicholas Allan, Merle Olson. 2024. Assessment of the Pharmacokinetics and Pharmacodynamics of Injectable Lidocaine and a Lidocaine-Impregnated Latex Band for Castration and Tail Docking in Lambs. Animals. 14(2):255.

· Denis Nagel, Brenda Ralston , Andrea Hanson, Les Burwash, Heather Matheson-Bird, Barbara Olson, Crystal Schatz, Merle Olson. 2024. Efficacy and Safety of Lidocam Topical Gel (4% Lidocaine—0.3% Meloxicam) for Pain and Inflammation Management during Castration and Tail Docking in Piglets. Animals. 2024(14):930.

· Manju Kundu, Amin Omar, Brenton Buziak, Nick Allan, Lyriam Marques, Merle Olson, Ronald Howard, Michael W. Harding. 2024. Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants — Their Two- and Three-Way Interactions. Applied Microbiology. 2024(4):27-46.

· Amritha Prasad, M. S. Roopesh. 2024. Bacterial biofilm reduction by 275 and 455 nm light pulses emitted from light emitting diodes. Journal of Food Safety 43.4 (2023): e13049.

· Gautam Gaur, Michael Gänzle. 2024. Biochemical characterization of HcrF from Limosilactobacillus fermentum, a NADH-dependent 2-ene reductase with activity on hydroxycinnamic acids. Letters in Applied Microbiology 77.12 (2024): ovae109.

· Walter Verhoef, Sjoert Zuidhof, Joseph A. Ross, Kendall Beaugrand, Merle Olson. 2023. Evaluation of a Novel Dipotassium Phosphate Bolus for Treatment of Metabolic Disorders in Dairy Cattle. Frontiers in Veterinary Science. 2023(10).

· Alysha JN. Burnett, Emily Rodriguez, Shirley Constable, Brian Lowrance, Michael Fish, Joel T. Weadge. 2023. WssI from the Gram-negative bacterial cellulose synthase is an O-acetyltransferase that acts on cello-oligomers with several acetyl donor substrates. Journal of Biological Chemistry 299(7):104849.

· Gautam Gaur, Michael G. Gänzle. 2023. Conversion of (poly) phenolic compounds in food fermentations by lactic acid bacteria: Novel insights into metabolic pathways and functional metabolites. Current Research in Food Science 6 (2023): 100448.

· Gautam Gaur, and Michael G. Gänzle. 2023. Role of thiols and ascladiol production in patulin degradation by lactobacilli. Letters in Applied Microbiology 76.3 (2023): ovad037.

· Gautam Gaur, Chen Chen, Michael G. Gänzle. 2023. Characterization of isogenic mutants with single or double deletions of four phenolic acid esterases in Lactiplantibacillus plantarum TMW1. 460. International Journal of Food Microbiology 388 (2023): 110100.

· Gautam Gaur, Sandra Damm, Maike Passon, Hiu Kwan Lo, Andreas Schieber, Michael G. Gänzle. 2023. Conversion of hydroxycinnamic acids by Furfurilactobacillus milii in sorghum fermentations: impact on profile of phenolic compounds in sorghum and on ecological fitness of Ff. milii. Food Microbiology 111 (2023): 104206.

· Paola Solis Ares, Gautam Gaur, Benjamin P. Willing, Fabian Weber, Andreas Schieber, Michael G. Gänzle. 2023. Antibacterial and enzyme inhibitory activities of flavan-3-ol monomers and procyanidin-rich grape seed fractions. Journal of Functional Foods 107 (2023): 105643.

· Nanzhen Qiao, Gautam Gaur, Monica Modesto, Fabio Chinnici, Donatella Scarafile, Luigimaria Borruso, Antonio Castro Marin, Caterina Spiezio , Daria Valente , Camillo Sandri , Michael G. Gänzle, Paola Mattarelli. 2023. Physiological and genomic characterization of Lactiplantibacillus plantarum isolated from Indri indri in Madagascar. Journal of Applied Microbiology 134, no. 11 (2023): lxad255.

· Benjamin C.T. Bourrie, Natalie Diether, Ryan P. Dias, Seo Lin Nam, A. Paulina de la Mata, Andrew J. Forgie, Gautam Gaur, James J. Harynuk, Michael Gänzle, Paul D. Cotter, Benjamin P. Willing. 2023. Use of reconstituted kefir consortia to determine the impact of microbial composition on kefir metabolite profiles." Food Research International 173 (2023): 113467.

· Steven M. Roche, Joseph A. Ross, Crystal Schatz, Kendall Beaugrand, Sjoert Zuidhof, Brenda Ralston, Nick Allan, Merle Olson. 2023. Impact of dystocia on milk production, somatic cell count, reproduction and culling in Holstein dairy cows. Animals. 13(3):346.

· Amritha Prasad, Michael Gänzle, M. S. Roopesh. 2023. Understanding the Salmonella inactivation mechanisms of 365, 395 and 455 nm light pulses emitted from light-emitting diodes. Applied Sciences 13.3 (2023): 1501.

· Michael W. Harding , Lyriam L. R. Marques, Nick Allan, Merle E. Olson, Brenton Buziak, Patricia Nadworny, Amin Omar, Ronald J. Howard, Jie Feng. 2022. Bactericidal Efficacy of Oxidized Silver against Biofilms Formed by Curtobacterium flaccumfaciens pv. Flaccumfaciens. The Plant Pathology Journal. 38(4):334-344.

· Pswarayi Felicitas, Nanzhen Qiao, Gautam Gaur, Michael Gänzle. 2022. Antimicrobial plant secondary metabolites, MDR transporters and antimicrobial resistance in cereal-associated lactobacilli: is there a connection? Food Microbiology 102 (2022): 103917.

· Joseph A. Ross, Crystal Schatz, Kendall Beaugrand, Sjoert Zuidhof, Brenda Ralston, Nick Allan, Merle Olson. 2021. Evaluation of Activated Charcoal as an Alternative to Antimicrobials for the Treatment of Neonatal Calf Diarrhea. Veterinary Medicine: Research and Reports. 2021(12):359-369.

· Walter Verhoef, Sjoert Zuidhof, Brenda Ralston, Joseph A. Ross, Merle Olson. 2021. Dissolution rates of calcium boluses and their effects on serum calcium in dairy cattle. Veterinary Medicine: Research and Reports. 2021(12):23-32.

· Amritha Prasad, Michael Gänzle, M. S. Roopesh. 2021. Antimicrobial activity and drying potential of high intensity blue light pulses (455 nm) emitted from LEDs. Food Research International 148 (2021): 110601.

· Abdullahi M. Adam, Barun Yadav, Amritha Prasad, Bina Gautam, Ying Tsui, M. S. Roopesh. 2021. Salmonellainactivation and rapid cooling of fresh cut apples by plasma integrated low-pressure cooling. Food Research International 147 (2021): 110464.

· William Scott, Brian Lowrance, Alex C. Anderson, Joel T. Weadge. 2020. Identification of the Clostridial cellulose synthase and characterization of the cognate glycosyl hydrolase, CcsZ. PLoS One 2:15(12):e0242686.

· James W. Saville, Joseph A. Ross, Tyler Trefz, Crystal Schatz, Heather Matheson-Bird, Brenda Ralston, Ori Granot, Karin Schmid, Richard Terry, Nick Allan, Jeremy E. Wulff, Merle Olson. 2020. Development and Field Validation of Lidocaine-Loaded Castration Bands for Bovine Pain Mitigation. Animals. 10(12):2363.

· Kamiko R. Bressler*, Joseph A. Ross*, Slava Ilnytskyy, Keiran Vanden Dungen, Katrina Taylor, Kush Patel, Athanasios Zovoilis, Igor Kovalchuk, Nehal Thakor. 2020. Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK). Cell Stress and Chaperones. 26(1):253-264. *Co-authors.

· Joseph A. Ross, Nick Allan, Merle Olson, Crystal Schatz, Patrick Nick Nation, Justin Peter Gawaziuk, Japandeep Sethi, Song Liu, Sarvesh Logsetty. 2020. Comparison of the efficacy of silver-based antimicrobial burn dressings in a porcine model of burn wounds. Burns. 46(7):1632-1640.

· Daniel Shock, Steven Roche, Denis Nagel, Merle Olson. 2020. The Effect of Delivery Method on the Pharmacokinetic Properties of Meloxicam in Pre-Weaned Dairy Calves with Diarrhea. Open Journal of Veterinary Medicine. 2020(10):27-38.

· Cassidy L. Klima, Devin B. Holman, Shaun R. Cook, Cheyenne C. Conrad, Brenda J. Ralston, Nick Allan, R. Michele Anholt, Yan D. Niu, Kim Stanford, Sherry J. Hannon, Calvin W. Booker, Tim A. McAllister. 2020. Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016. Frontiers in microbiology. 11(2020):606438.

· Amritha Prasad, Lihui Du, Muhammad Zubair, Samir Subedi, Aman Ullah, M. S. Roopesh. 2020. Applications of light-emitting diodes (LEDs) in food processing and water treatment. Food Engineering Reviews 12 (2020): 268-289.

· Lihui Du, Amritha Jaya Prasad, Michael Gänzle, M. S. Roopesh. 2020. Inactivation of Salmonella spp. in wheat flour by 395 nm pulsed light emitting diode (LED) treatment and the related functional and structural changes of gluten. Food Research International 127 (2020): 108716.

· Samir Subedi, Lihui Du, Amritha Prasad, Barun Yadav, M. S. Roopesh. 2020. Inactivation of Salmonella and quality changes in wheat flour after pulsed light-emitting diode (LED) treatments. Food and Bioproducts Processing 121 (2020): 166-177.

· Gautam Gaur, Jee-Hwan Oh, Pasquale Filannino, Marco Gobbetti, Jan-Peter Van Pijkeren, Michael G. Gänzle. 2020. Genetic determinants of hydroxycinnamic acid metabolism in heterofermentative lactobacilli. Applied and environmental microbiology 86, no. 5 (2020): e02461-19.

· Joseph A. Ross, Bo Young Ahn, Jennifer King, Kamiko R. Bressler, Donna L. Senger, Nehal Thakor. 2019. Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumor stem cells (BTSCs). Biochemistry and Cell Biology. 98(6):647-652.

· Jean Claud Nshogozabahizi, Keith Aubrey, Joseph A. Ross*, Nehal Thakor. 2019. Applications and limitations of regulatory RNA elements in synthetic biology and biotechnology. Journal of Applied Microbiology. 127(4):968-984. (Review.) *Co-corresponding author.

· Nirujah Balasingam*, Harland Brandon*, Joseph A. Ross*, Hans-Joachim Wieden, Nehal Thakor. 2019. Cellular roles of the human Obg-like ATPase 1 (hOLA1) and its YchF homologs. Biochemistry and Cell Biology. 98(1):1-11. (Review.) *Co-authors.

· Joseph A. Ross, Mette Thorsing, Eva Maria Sternkopf Lillebaek, Patrícia Teixeira dos Santos, Birgitte H. Kallipolitis. 2019. The LhrC sRNAs control expression of T cell-stimulating antigen TcsA in Listeria monocytogenes by decreasing tcsA mRNA stability. RNA Biology. 16(3):270-81.

· Joseph A. Ross, Keiran Vanden Dungen, Kamiko R. Bressler, Mikayla Fredriksen, Divya K. Sharma, Nirujah Balasingham, Nehal Thakor. 2019. Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis. Cell Death and Disease. 10(2):57.

· Amritha Prasad, Michael Gänzle, M. S. Roopesh. 2019. Inactivation of Escherichia coli and Salmonellausing 365 and 395 nm high intensity pulsed light emitting diodes. Foods 8.12 (2019): 679. 

· Joseph A. Ross, Kamiko Bressler, Nehal Thakor. 2018. Eukaryotic initiation factor 5B (eIF5B) cooperates with eIF1A and eIF5 to facilitate uORF2-mediated repression of ATF4 translation. International Journal of Molecular Sciences. 19(12):4032.

· Joseph A. Ross and Nehal Thakor. 2018. Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs. Invited submission to JoVE.(135):57519. doi: 10.3791/57519.

· R. Michele Anholt, Cassidy Klima, Nick Allan, Heather Matheson-Bird, Crystal Schatz, Praseeda Ajitkumar, Simon JG Otto, Delores Peters, Karin Schmid, Merle Olson, Tim McAllister, Brenda Ralston. 2017. Antimicrobial Susceptibility of Bacteria That Cause Bovine Respiratory Disease Complex in Alberta, Canada. Frontiers in Veterinary Science. 4:207.

· Maria S. Mollerup, Joseph A. Ross, Anne-Catherine Helfer, Kristine Meistrup, Pascale Romby, Birgitte H. Kallipolitis. 2016. Two novel members of the LhrC family of small RNAs in Listeria monocytogenes with overlapping regulatory functions but distinctive expression profiles. RNA Biology. 13(9):895-915.

· Merle E. Olson, Denis Nagel, Sherry Custead, Waylon Wise, Kirby Penttila, Les Burwash, Brenda Ralston, Crystal Schatz, Heather Matheson-Bird. 2016. The Palatability and Comparative Efficacy of Meloxicam Oral Suspension for the Treatment of Chronic Musculoskeletal Disease in Horses. Journal of Equine Veterinary Science. 44(2016):26-31. 

· Merle E. Olson, Brenda Ralston, Les Burwash, Heather Matheson-Bird, Nick D. Allan. 2016. Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves. BMC Veterinary Research. (2016)12:102.

· Weng Xuan, Gautam Gaur, Suresh Neethirajan. 2016. Rapid detection of food allergens by microfluidics ELISA-based optical sensor. Biosensors 6.2 (2016): 24.

· Joseph A. Ross, Edward Topp. 2015. Abundance of Antibiotic Resistance Genes in Bacteriophage following Soil Fertilization with Dairy Manure or Municipal Biosolids, and Evidence for Potential Transduction. Applied and Environmental Microbiology. 81(22):7905-13.

· Michael J. Ellis, Ryan S. Trussler, Joseph A. Ross, David B. Haniford. 2015. Probing Hfq:RNA interactions with hydroxyl radical and RNase footprinting. Chapter in: RNA remodeling proteins: methods and protocols. Marc Boudvillain, Ed. Methods in Molecular Biology, vol. 1259. Springer Science+Business Media New York. January 2015.

· Merle E. Olson, Erin Fierheller, Les Burwash, Brenda Ralston, Crystal Schatz, Heather Matheson-Bird. 2015. The Efficacy of Meloxicam Oral Suspension for Controlling Pain and Inflammation After Castration in Horses. Journal of Equine Veterinary Science. 35(2015):724-730.

· Samarajeewa, AD, Hammad, A, Masson, L, Khan, IUH, Scroggons, R and Beaudette, LA. 2015. Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia. Journal of Microbiological Methods. Jan;108:103-111.

· Joseph A. Ross, Ryan S. Trussler, Morgan D. Black, Crystal R. McLellan, David B. Haniford. 2014. Tn5 transposition in Escherichia coli is repressed by Hfq and activated by over-expression of the small non-coding RNA SgrS. Mobile DNA. 5(1):27. 

· Allan E. Parker, D.K. Walker, Darla M. Goeres, Nick Allan, Merle E. Olson, A. Omar. 2014. Ruggedness and reproducibility of the MBEC biofilm disinfectant efficacy test. Journal of Microbiological Methods. 102:55-64.

· Joseph A. Ross, Michael J. Ellis, Shahan Hossain and David B. Haniford. 2013. Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10system. RNA. 19(5):670-684. 

· Nicholas D. Allan, Kamna Giare-Patel, Merle E. Olson. 2012. An In Vivo Rabbit Model for the Evaluation of Antimicrobial Peripherally Inserted Central Catheter to reduce Microbial Migration and Colonization as Compared to an Uncoated PICC. Journal of Biomedicine and Biotechnology. 2012(1):921617.

· M.W. Harding, R.J. Howard, G.D. Daniels, S.L. Mobbs, S.L.I. Lisowski, N.D. Allan, A. Omar, M.E. Olson. 2011. A multi-well plate method for rapid growth, characterization and biocide sensitivity testing of microbial biofilms on various surface materials. Chapter in: Science against microbial pathogens: communicating current research and technological advances. A. Méndez-Vilas, Ed. pp.872-877. 

· N.D. Allan, A. Omar, M.W. Harding, M.E. Olson. 2011. A rapid, high-throughput method for culturing, characterizing and biocide efficacy testing of both planktonic cells and biofilms. Chapter in: Science against microbial pathogens: communicating current research and technological advances. A. Méndez-Vilas, Ed. pp. 864-871.

· Joseph A. Ross, Simon J. Wardle and David B. Haniford. 2010. Tn10/IS10transposition is down-regulated at the level of transposase expression by the RNA-binding protein Hfq. Molecular Microbiology. 78(3):607-21.

· Joe J. Harrison, Carol A. Stremick, Raymond J. Turner, Nick D. Allan, Merle E. Olson, Howard Ceri. 2010. Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening. Nature Protocols. 5(7):1236-1254.

· Joe J. Harrison, Raymond J. Turner, Daniel A. Joo, Michelle A. Stan, Catherine S. Chan, Nick D. Allan, Helen A. Vrionis, Merle E. Olson, and Howard Ceri. 2008. Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy. 52(8):2870-2881.

· Marin H. Kollef, Bekele Afessa, Antonio Anzueto, Christopher Veremakis, Kim M. Kerr, Benjamin D. Margolis, Donald E. Craven, Pamela R. Roberts, Alejandro C. Arroliga, Rolf D. Hubmayr, Marcos I. Restrepo, William R. Auger, Regina Schinner. 2008. Silver-Coated Endotracheal Tubes and Incidence of Ventilator-Associated Pneumonia: The NASCENT Randomized Trial. JAMA. 300(7):805-813.

· Merle E. Olson, Barry G. Harmon, Marin H. Kolle. 2002. Silver-Coated Endotracheal Tubes Associated With Reduced Bacterial Burden in the Lungs of Mechanically Ventilated Dogs. Chest. 121(3):863-870.

Standards:

· E2799-22: Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay. ASTM International. Originally published 2011.

· E3435-25: Standard Practice for Testing Antimicrobial or Disinfectant Efficacy Against Biofilms Grown on a Medical Device or Surface by the Biofilm Surface Test Protocol (BSTP). ASTM International. Originally published 2024.